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KMID : 0881720220370020046
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Journal of Food Hygiene and Safety
2022 Volume.37 No. 2 p.46 ~ p.54
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Development of Method using LC-ESI-MS/MS and KASP for Identification of Gymnema sylvestre in Food
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Park Bo-Reum
Lee Sun-Hee
Eom Kwon-Yong
Noh Eun-Young
Han Kyoung-Moon
Hwang Jin-Woo
Kim Hyung-Il
Baek Sun-Young
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Abstract
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Known for its effectiveness in weight loss and diabetes prevention, Gymnema sylvestre products can be found in the US, Japanese, and Indian markets. However, the recommended dosage or safety of these products has not yet been proven. Therefore, development of an analytical method for detecting the content of Gymnema sylvestre in food products is required. Accordingly, this study proposes an analysis method that can examine Gymnema sylvestre in food using LC-ESI-MS/MS and KASP (Kompetitive Allele-Specific PCR) markers. In LC-ESI-MS/MS, a simultaneous analysis method for gymnemic acid and deacylgymnemic acid was optimized using negative ionization mode, and its validation test was completed for solid and liquid samples. In addition, KASP markers were prepared by finding the specific SNP of Gymnema sylvestre in ITS2 and matK through DNA barcodes. The two KASP markers returned positive FAM fluorescence result when combined with Gymnema sylvestre, and this aspect was confirmed in raw Gymnema sylvestre as well. The applicability of the method was tested on 21 different food and healthy functional products containing Gymnema sylvestre purchased on the internet. As a result, although there was a difference in the ratios of gymnemic acid and deacylgymnemic acid in LC-ESI-MS/MS, the index component was detected in all 21 products samples. In the KASP analysis, 9 products returned positive FAM result, and the rest of the products were found to be containing Gymnema sylvestre extract. This study is the first study to use the dual system of LC-ESIMS/MS and KASP for the analysis of Gymnema sylvestre. The study has confirmed that these two methods are applicable to the examine Gymnema sylvestre content in food products.
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KEYWORD
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Gymnema sylvestre, LC-ESI-MS/MS, KASP, Food analysis
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